From the follicular fluid obtained from the egg pick up procedure , mature eggs are picked up and after specific incubation period in side the Tri gas Incubators, either conventional IVF or ICSI is planned. For culturing embryos until Day 5 , we need three essential gases – Oxygen, Carbon di Oxide and Nitrogen used in Bench top incubators , in the culture medium containing all essential nutrients to nourish and help to grow the embryos in stages. Specific Mouse embryo tested utensils are used in the lab for embryo culture which are imported from UK, USA and Australia.
The culture dishes are kept in a modular chamber which holds the gaseous environment very strictly and these are kept in turn in a CO2 incubator. Our IVF lab utilizes TRIGAS (O2, CO2, N2 mixture) incubator instead of CO2 alone , since the presence of 5 % O2 improves the culture status and integrity of embryos.
After 48 hours the cell division shows 4 cells inside the embryos and after 72 hours they show 8 cell divisions. Depending on the number of 8 cell stage the viability of the culture system is assessed. The success rates are also decided according to this.
